Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 3;42(8):4996–5006. doi: 10.1093/nar/gku160

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Loss of K-H leads to increased DSBs and genomic instability. (A) shScr, shk-h, shScr-MDA231, shk-h-MDA231, mk-h^+/+ and mk-h^+/– cells were used to interrogate the degree of K-H loss each cell system. (B and C) Basal levels of the DNA damage indicator γ-H2AX were measured in shScr-MDA231, shk-h-MDA231, mk-h^+/+ and mk-h^+/– cells by immunofluorescence (IF). (D and E) Rates of γ-H2AX disappearance were measured in shScr-MDA231, shk-h-MDA231, shk-h-MDA231 + K-H, mk-h^+/+ and mk-h^+/– cells at indicated times after ionizing radiation (IR) exposure by IF, the first time point is 0.5 h after IR exposure. (F) Amounts of genomic aberrations were quantitated in shScr and shk-h cells with and without exposure to IR (2 Gy). (**P < 0.01).