Table 2.
Theoretical molecular weights (Da) of various subunit stoichiometries of EcoP15I, the biotinylated EcoP15I and EcoPI in 1:1 and 1:2 complex with Hex-labelled or unlabelled 50-mer specific DNA
| Hex-labelled 50-mer |
unlabelled 50-mer |
||||
|---|---|---|---|---|---|
| 1:1 | 1:2 | 1:1 | 1:2 | ||
| EcoP15I | R1M2 | 290 658 | 322 171 | 289 914 | 320 683 |
| R2M2 | 401 615 | 433 128 | 400 871 | 431 640 | |
| M2 | 179 701 | 211 214 | 178 957 | 209 726 | |
| bio-EcoP15I | R1M2 | 292 989 | 324 502 | 292 245 | 323 014 |
| R2M2 | 406 277 | 437 790 | 405 533 | 436 302 | |
| M2 | 179 701 | 211 214 | 178 957 | 209 726 | |
| EcoPI | R1M2 | 289 944 | 321 457 | 289 200 | 319 969 |
| R2M2 | 401 403 | 432 916 | 400 659 | 431 428 | |
| M2 | 178 485 | 209 998 | 177 741 | 208 510 | |