Table 3.
Molecular weights (kDa) of free and 50-mer specific DNA complexed Type III REs in the absence and presence of ATP as measured by SEC-MALS experiments
| − ATP |
+ ATP |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| no DNA | 1:0.5 | 1:1 | 1:2 | 1:4 | no DNA | 1:0.5 | 1:1 | 1:2 | 1:4 | |
| EcoP15I | 264 | 304 | 306 | 311 | 306 | 267 | 279 | 274 | 280 | |
| 373b | 377b | |||||||||
| EcoP15I NEB | 269 | |||||||||
| bioEcoP15I | 266 | |||||||||
| R534A EcoP15I | 270 | 294 | 269 | 300 | ||||||
| EcoPI | 273 | 283 | 289 | 291 | 293 | 268 | 277 | 275 | ||
| PstII | 132a | |||||||||
| 205a | ||||||||||
aEquilibrium between two species.
bEquilibrium between multiple species, average molecular weight of multiple species due to sample heterogeneity.