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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Mol Microbiol. 2014 Mar 19;92(2):383–398. doi: 10.1111/mmi.12565

Figure 3.

Figure 3

Pheromone signaling is necessary, but not sufficient, for sexual biofilm formation by C. tropicalis opaque cells. (A) Quantification of biofilm formation by C. tropicalis strains lacking pheromone receptors for α pheromone (Ste2) or for a pheromone (Ste3). * p<0.001. Mann-Whitney test. (B) Biofilm formation by C. tropicalis a cells supplemented with synthetic α pheromone at 100 μg/ml (13-mer, 15-mer or 16-mer) or α cells. ° p<0.05 compared to a cells alone. * p<0.01 compared to a cells alone. Mann-Whitney test. (C) Biofilm formation of a cells (sink cells) exposed to pheromones produced by a mixed culture of opaque a and α cells (source cells), that were separated from sink cells by a semi-permeable transwell. * p<0.001, Tukey’s test. (D) Schematic diagram of transwell setup. Biofilm measurements show mean ± standard deviation (N≥4).