Table 2.

Enzyme amounts used

	Enzyme	Substrate	Coupling Enzyme	OD	Units/mg	mg added	Units added
1	GsPyk	PEP/ADP	LDHa	340	55.2 ± 0.08	0.012	0.66
2	GsPDH	Pyruvate/CoA/NAD+	none	340	153.4 ± 0.01b	–	0.038
3	LlNoxE	NADH	none	340	9.0 ± 0.002	0.021	0.19
4	EfTHL-HMGR	Acetyl-CoA/NADPH	EfHMGS	340	0.06 ± 0.002c	0.021	0.0013
5	EfHMGSd	Acetyl-CoA/Acetoacetyl-CoA	none	303	0.6 ± 0.01	0.014	0.0084
6	ScHMGR-t	HMG-CoA	none	340	0.5 ± 0.08	0.012	0.0060
7	ScMVK	Mevalonate/ATP	PK/LDHa	340	47.0 ± 0.9	0.003	0.14
8	SsPMVK	Mevalonate-P/ATP	PK/LDHa	340	0.8 ± 0.02	0.013	0.011
9	ScMDC	Mevalonate-PP/ATP	PK/LDHa	340	4.0 ± .07	0.012	0.048
10	EcIDI	ND			0.035e	0.028	0.00098
11	PaIspS	ND			0.156f	0.016	0.0025
12	G6PDHa	ND			200-400c	0.005	1.0

^aFrom Sigma.

^bUnits/mL.

^cAssayed in forward direction (synthesis) by coupling to MvaS. NADPH consumption monitored at 340 nm.

^dMutant A110G (From Ref. 30).

^eFrom Ref. 35.

^fFrom Ref. 36.