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. 2014 Apr 30;9(4):e95136. doi: 10.1371/journal.pone.0095136

Figure 3. Loss of SSX expression arrest tumor cells in G1/S phase and abrogates -cyclin E expression.

Figure 3

Control (SSX+) and silenced (SSX−) DFW melanoma cells were synchronized in G1/S phase by double thymidine blockade and released in normal medium as described in material and methods. A) Entry of the cells to the S phase (S) was analyzed by quantifying DNA content using propidium iodide (PI) incorporation and a fluorescent activated cell sorter FACS. B) Western blot showing time dependent expression of SSX and cyclin E in control and SSX silenced (+Dox) DFW cells. Cells were syncronized in G1/S (0 h), released into normal medium containing FBS and harvested at the indicated time points.