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. 2014 Apr 30;9(4):e95136. doi: 10.1371/journal.pone.0095136

Figure 4. SSX is required for Erk mediated signalling.

Figure 4

Western blot showing the expression of Erk1–2 and Akt-1 and their activated (phosphorylated) forms pAkt (Ser 473) and pErk (Thr202/Tyr204) in control shRNA and SSX-shRNA DFW cells. Cells were starved in serum free media for 36 hrs (0 h) and cell signalling was activated by the addition of serum into the media. Samples were collected at 10 and 30 minutes (10′ and 30′) and at 6 and 24 hours (6 h, 24 h) following serum stimulation. SSX expression was determined by immunoprecipitation (fl188 antibody) and western blot (N18 antibody) the later recognozing bands of approximately 22 and 14 kDa.