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. 2014 Apr 30;9(4):e95884. doi: 10.1371/journal.pone.0095884

Figure 1. IL-6 promotes lung carcinoma cell invasion and EMT.

Figure 1

(A) A549 and HCC827 cell mesenchymal phenotype induced by IL-6 (200×). (B) A549 and HCC827 cell invasion in the presence of various concentrations of IL-6. (C) E-cadherin, vimentin and snail protein levels were examined by Western blotting. Equal protein loading was verified by β-actin. (D) mRNA expression levels of E-cadherin, vimentin and snail were analyzed by quantitative PCR and normalized by GAPDH. (E) E-cadherin and vimentin protein levels were detected by immunofluorescence (800×). Error bars represent the standard deviation. *, p<0.05, **, p<0.005.