2D-MRI images and H&E-stained horizontal sections of the BBB-opened hippocampi at (a-c) 0.15 MPa, (d-f) 0.30 MPa, (g-i) 0.45 MPa and (j-l) 0.60 MPa using transducer-A and microbubbles. Sonicated brains at 0.15 MPa, 0.30 MPa and 0.45 MPa showed no histological damage. Brain samples sonicated at 0.60 MPa (j)-(l) showed higher incidence of microscopic damage at multiple distinct damaged sites. The black arrows point to the RBC extravasations. The black boxes in the left and middle columns indicate the enlarged regions shown in the middle column and right column, respectively. Magnifications and scale bars in (a), (d), (g) and (j) are 40× and 200 μm, in (b), (e), (h) and (k) 100× and 100 μm and in (c), (f), (i) and (l) 200× and 50 μm, respectively.