TABLE 5.
Effect of the addition of thymidine analogs to a TK assaya
| Nucleoside added | % Activityb |
|---|---|
| None | 100 |
| dThd | 9.1 ± 3.2 |
| AZT | 5.4 ± 2.3 |
| D4T | 106.3 ± 7.7 |
| 4′-Methyl D4T | 103.8 ± 9.9 |
| 4′-Vinyl D4T | 99.5 ± 6.9 |
| 4′-Ethynyl D4T | 83.9 ± 6.1 |
| 4′-Methylethynyl D4T | 74.0 ± 7.9 |
| 4′-Chloroethynyl D4T | 53.2 ± 9.2 |
| 4′-Allyl D4T | 110.8 ± 9.0 |
| 4′-Cyano D4T | 71.8 ± 8.2 |
a
The assays were performed essentially as described in Materials and Methods except that the [14C]dThd concentration in the assay was reduced to 25 μM and the concentration of the added nucleoside was 250 μM.
b
Numbers represent means and standard deviations of six to eight determinations.