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. Author manuscript; available in PMC: 2014 Apr 30.
Published in final edited form as: Nat Immunol. 2012 Aug 26;13(10):954–962. doi: 10.1038/ni.2397

Figure 1.

Figure 1

Prolonged survival of Ifnar1−/− mice to S. Typhimurium infection. (a–c) Survival (a) and bacterial burden in the spleen (b,c) of wild-type (WT) and Ifnar1−/− mice infected intravenously with 1 × 102 S. Typhimurium (ST; a,b) or intraperitoneally with 1 × 103 S. Typhimurium (c). † indicates death. CFU, colony-forming units. (d) Bacterial burden in the spleens of lethally irradiated wild-type or Ifnar1−/− recipient mice given bone marrow cells (1 × 107) from wild-type or Ifnar1−/− donors, then challenged 3 months later by intravenous infection with 1 × 102 S. Typhimurium and assessed at day 5 after infection. (e,f) Flow cytometry of cells in the spleens of wild-type and Ifnar1−/− mice 5 d after intravenous infection with 1 × 102 S. Typhimurium. Arrows and outlined areas in e indicate CD11b+Gr-1− cells (top row) or CD11b+F4/80+ cells (bottom row). (g) Bacterial burden in the spleens of naive wild-type mice given intravenous injection of bone marrow–derived macrophages from wild-type or Ifnar1−/− mice (5 × 106 cells), then challenged the next day by intraperitoneal infection with 1 × 102 S. Typhimurium and assessed 5 d after infection. (h) Intracellular survival of S. Typhimurium in bone marrow–derived macrophages after infection for 30 min in vitro (multiplicity of infection (MOI), 10). **P < 0.01 (one way ANOVA). Data are representative of two experiments with similar results, with three to five mice per group (mean and s.e.m. d,f–h).