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. Author manuscript; available in PMC: 2014 Apr 30.
Published in final edited form as: Science. 2013 Sep 26;342(6157):447–453. doi: 10.1126/science.1237910

Fig. 5. Galectin-3, Dectin-1 and FcγRIIB enhance gut homeostasis and oral tolerance.

Fig. 5

(A) FC of galectin-3, FcγRIIB and Dectin-1 on SI-LP DCs from WT mice. MFI, mean fluorescence intensity. (B) Quantification of MUC2+ SI-LP DCs from WT, Lgals3−/−, Clec7A−/− or Fcgr2b−/− mice by IFA of 10–12 SI-LP sections/group. (C) qRT-PCR of Muc2 in IECs and SI-LP DCs from WT mice. RE, relative expression compared to Gapdh. (D and E) qRT-PCR of Il12a, Il12b, Aldh1a1 and Tgfb1 in SI-LP DCs and FC of Foxp3 and IFN-γ in SI-LP CD4+ T cells from WT, Lgals3−/−, Clec7A−/− or Fcgr2b−/− mice. RE, relative expression compared to Gapdh. (F) DTH and ELISA of OVA-specific IgE in WT, Lgals3−/−, Clec7A−/− or Fcgr2b−/− mice intragastrically tolerized with PBS or OVA for 5 days and subcutaneously immunized with OVA. (G) ELISA of proliferation-induced BrdU from SPL CD4+ T cells activated for 5 days by OVA-pulsed SPL DCs from WT, Lgals3−/−, Clec7A−/− or Fcgr2b−/− mice tolerized and immunized as in (F). Data summarize 2 experiments with ≥4 mice/group (error bars, s.d.; unpaired Student’s t test, *P <0.05) or show one of 4 experiments with similar results.