Figure 7. Mouse splenic ILCs help plasmablasts and plasma cells emerging from TI IgG3 responses.

(a) Flow cytometric analysis of frequency and absolute numbers of splenic Lin^–CD117⁺CD127⁺ ILCs from Thy-1-disparate chimeric Rag1^–/– mice treated with control (ctrl) (n = 7) or anti-Thy.1.2 antibodies (n = 7). (b) Frequency and absolute numbers of splenic IgG3E⁺IgG3I^lo B cells, IgG3E^hiIgG3I⁺ plasmablasts (PBs) and IgG3E^–IgG3I^hi plasma cells (PCs) from Ctrl or ILC-depleted mice obtained as in (a). E, extracellular; I, intracellular. (c,d) ELISA of pre-immune total (c) and PC-reactive (d) serum IgG3 from Ctrl or ILC-depleted mice obtained as in (a). (e,f) Frequency and absolute numbers of splenic Lin^– CD117⁺CD127⁺ ILCs (e) and IgG3-expressing splenic B cells, PBs and PCs (f) from ILC⁺ (n = 3) or ILC^– (n = 3) bone marrow chimeric mice. (g) Pre-immune total serum IgG3 from ILC⁺ (n = 4) and ILC^– (n = 3) mice, age-matched Rorc^+/+ mice (n = 10), and Rorc^–/– mice (n = 5). Error bars, s.d.; *P <0.05 (one-tailed unpaired Student's t test). Data show one of four experiments with similar results (a,b,e,f: cytograms) or summarize results from 3-10 animals per each group (a,b,e,f: bars; c,d,g).