Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 28;9:64. doi: 10.1186/1750-1172-9-64

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Kwiatkowska et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Selective recognition of cholesterol by GST-PFO. (A) Protein-lipid overlay assay. Indicated amounts of lipids were spotted on nitrocellulose membrane and incubated with 1 μg/ml GST-PFO. Immunoreactive spots were revealed by chemiluminescence. (B) 6-Carboxyfluorescein release from liposomes of indicated composition induced by 15 μg/ml GST-PFO. The data are expressed as percentage of the total amount of 6-carboxyfluorescein released from liposomes by 0.2% Triton X-100. (C) SPR sensograms of binding of 10 μg/ml GST-PFO or 20 μg/ml GST to liposomes of indicated lipid composition immobilized on the surface of sensor chip L1. Binding of GST-PFO to liposomes was carried out for 600 s after which the samples were washed for another 600 s. In (A) and (C) data represent one of three independent experiments; in (B) mean ± SEM from three experiments is shown. Chol – cholesterol; Cer – ceramide; SM – sphingomyelin.