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Quantitation of colocalization of GST-PFO-positive structures and other cellular components in NPC fibroblasts. (A) Pearson’s correlation coefficient, (B) overlap coefficient for co-immunostaining with GST-PFO and filipin or antibodies against LAMP1, LBPA, golgin-84, PDI, PMP70 or phalloidin used as an actin filament marker. TX, Triton X-100, DiT, digitonin. Data shown are mean ± SEM.
