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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Bioorg Med Chem Lett. 2014 Feb 7;24(6):1538–1544. doi: 10.1016/j.bmcl.2014.01.079

Figure 3. Crosslinking trials using azide probe 27.

Figure 3

GAS cultures were grown in the presence of 27 with or without competitor 3, then subjected to UV crosslinking conditions. The cells were lysed and a fluorescent Alexa Fluor 488 moiety was appended to probe-protein adducts via click chemistry, then proteins were isolated and separated via SDS-PAGE. A: Fluorescent visualization of proteins from crosslinking and competition experiments with 27 on PAGE gel. B: Total protein stain of gel with Sypro Ruby stain. Lane 1: 100 μM 27; Lane 2: 10 μM 27; Lane 3: 10 μM 27 + 100 μM 3; Lane 4: 1 μM 27.