(A) Anesthetized WT and AMPK KD mice were treated with A-769662 (6 mg/kg IP) or vehicle, and hearts were excised after 20 min. Heart homogenates were immunoblotted for p-AMPK (Thr172), AMPKα, p-ACC (Ser79), and ACC. Representative blots and quantification of the ratios of phosphorylated to total protein content are shown (n=4-6/group). (B and C) Anesthetized WT and AMPK KD mice were treated with A-769662 (6 mg/kg IP) or vehicle 30 min prior to left coronary artery ligation (20 min) and reperfusion (4 hours). Representative dual stained heart sections and bar graphs quantifying the ratio of infarcted area (INF) to the area at risk (AAR) and the ratio of AAR to total left ventricular area (LV) are shown (n=5-7/group). (D) Additional hearts were fixed and apoptotic nuclei in the ischemic-reperfused region were identified by TUNEL staining (original magnification, x40) (n=4/group). White bar, vehicle. Black bar, A-769662. Values are expressed as mean + SEM. *P<0.05.