Figure 3.

Role of PP2A in PA-induced VEGF signaling defect. A: Coimmunoprecipitation of PP2A and eNOS, ERK, and Akt in BAECs. For densitometric quantification, the control ratio was defined as 100%. The reverse coimmunoprecipitations are presented in Supplemental Figure S1. B: Effect of PP2A inhibition on VEGF-induced eNOS-Ser¹¹⁷⁹ phosphorylation. BAECs were pretreated for 4 hours with 100 μmol/L PA or control in the presence or absence of 100 nmol/L PP2A inhibitor OkA. Cells were then treated with 100 ng/mL VEGF for 0, 5, or 20 minutes. For densitometric quantification of p-eNOS/total eNOS in VEGF 5-minute samples, the control ratio was defined as 100%. Data are expressed as means ± SEM. Blots and data are representative of three independent experiments. ^∗∗P < 0.01.