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. 2014 May;184(5):1562–1576. doi: 10.1016/j.ajpath.2014.01.009

Figure 8.

Figure 8

Effect of SPT haploinsufficiency on aortic ceramide levels and angiogenic responses. A and B: Accumulation of ceramide metabolites in aortas of Sptlc2−/+ (white bars) and Sptlc2+/+ (gray bars) mice fed normal chow (A) or a high-fat diet (B) was analyzed by LC-MS/MS. C–E: VEGF-induced activation of Akt and eNOS in aortas of Sptlc2−/+ and Sptlc2+/+ mice fed normal chow or a high-fat diet was analyzed by immunoblotting. Representative immunoblots (C) and densitometry analyses (D and E). F and G: AdVEGF-induced angiogenesis in the ears of high-fat-fed Sptlc2−/+ and Sptlc2+/+ mice. Representative images (F) and quantitative analyses (G) are shown. Data are expressed as means ± SD (A and B) or as means ± SEM (C–E and G). n = 3 mice per group. ∗P < 0.05, ∗∗∗P < 0.001. Original magnification, ×400.