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. 2004 May;186(10):2973–2983. doi: 10.1128/JB.186.10.2973-2983.2004

FIG. 1.

FIG. 1.

Western immunoblot of whole-cell extracts of P. aeruginosa K2274 (A) and E. coli SU101 (B), demonstrating expression of wild-type and mutant MexA proteins from mexA genes cloned onto pRK415 (A) and pMS604 (B). Lane 1, wild-type MexA; lane 2, vector control; lane 3, MexAP68S; lane 4, MexAG72S; lane 5, MexAA108T; lane 6, MexAV129 M; lane7, MexAT256I; lane 8, MexAV264E; lane9, MexAN270S; lane 10, MexAH279Y; lane 11, MexAV286D; lane 12, MexAG297D; lane 13, MexAL91P; lane 14, MexAL110P. See Table 1 for designations of the pMS604 and pRK415 derivatives carrying the indicated wild-type and mutant mexA genes.