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. 2004 May;186(10):2973–2983. doi: 10.1128/JB.186.10.2973-2983.2004

FIG. 3.

FIG. 3.

Western immunoblot demonstrating MexB-His-dependent recovery of MexA on Ni-NTA. P. aeruginosa strain K2275 carrying plasmid pDN3 (pRK415::mexA) and either pDN25 (pMMB206:: mexB-6his) (lanes 1 and 2) or pMMB206 (lanes 3 and 4) was treated with 4 mM DSP, and Triton X-100-soluble extracts of a cell envelope preparation were incubated with Ni-NTA. Triton X-100-soluble cell envelope extracts (1 μl) applied to Ni-NTA (lanes 1 and 3) and DTT-treated elution fractions (10 μl) recovered from Ni-NTA (lanes 3 and 4) were immunoblotted and developed with antibodies to MexA (A) and MexB (B).