Figure 10.

Lactacystin blocks acetaldehyde-mediated COL1A2 gene transcription, but not acetaldehyde-mediated fibronectin gene transcription. A and B: Lactacystin prevented acetaldehyde-mediated up-regulation of COL1A2 by 60% (P < 0.05) (A), but had no effect on the up-regulation of fibronectin transcription (B). Controls are assigned a value of 100%. All values were corrected for loading differences using an S14 ribosomal protein cDNA. Data are expressed as means ± SEM, representative of three independent experiments. ^∗P < 0.05 versus control. ^†P < 0.05 versus acetaldehyde-treated cells.