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. 2014 May;184(5):1458–1467. doi: 10.1016/j.ajpath.2014.01.020

Figure 2.

Figure 2

TGF-β1 is involved in late acetaldehyde-dependent up-regulation of COL1A2. Run-on transcription assays of control (white bars; untreated cells in serum-free medium) and acetaldehyde-treated (black bars) HHSCs for 24 hours in the presence or absence of a nonspecific IgG or a neutralizing antibody to TGF-β1 (gray bars; anti–TGF-β1). As a control for these experiments, some HSCs were treated with 2 ng/mL of TGF-β1 in the absence or presence of the neutralizing antibody to the cytokine. COL1A2 gene transcription was up-regulated 3.5-fold by acetaldehyde, whereas the neutralizing antibody to TGF-β1 strongly inhibited COL1A2 gene transcription in cells treated with acetaldehyde by 60%. The nonspecific immunoglobulin had no effect. Data are expressed as means ± SEM. ∗P < 0.05 versus control. †P < 0.05 versus acetaldehyde-treated cells.