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. 2014 May;184(5):1458–1467. doi: 10.1016/j.ajpath.2014.01.020

Figure 3.

Figure 3

Increase in SMAD3 and SMAD4 mRNA and protein is delayed after acetaldehyde treatment. A: Time-course analysis of SMAD3 and SMAD4 mRNA expression levels after acetaldehyde treatment. Total RNA obtained from HHSCs cultured in the absence or presence of 200 μmol/L acetaldehyde for times ranging from 30 minutes to 48 hours was used to determine steady-state levels of SMAD3 and SMAD4 mRNAs by using RT-PCR analysis. B: Replica dishes were used to obtain cell extracts and to determine levels of SMAD3 and SMAD4 protein by Western analysis. Data are expressed as means ± SEM. ∗P < 0.05 versus control (A); †P < 0.05 versus 30-minute time point (B).