Figure 6.

Acetaldehyde modifies cellular distribution of Ski. Immunofluorescence microscopy of mouse HSCs cultured in the absence or presence of 200 μmol/L acetaldehyde for 30 minutes. Cells were fixed and stained with a rabbit anti-Ski antibody followed by a Cy3 (red) labeled anti-rabbit immunoglobulin. Although fluorescence in control untreated cells was concentrated predominantly in the nuclei, in acetaldehyde-treated cells the intensity of the nuclear signal decreased and the cytosol signal increased. In addition, acetaldehyde-treated cells appear more granular and diffused (arrows). Original magnification, ×20.