Skip to main content
. 2014 May;184(5):1458–1467. doi: 10.1016/j.ajpath.2014.01.020

Figure 8.

Figure 8

Ski and SMAD4 colocalize in the nuclei of HSCs and translocate to the cytosol after acetaldehyde administration. A: Immunofluorescence microscopy of mouse HSCs cultured in the absence or presence of 200 μmol/L acetaldehyde for 30 minutes. Cells were stained with antibodies to SMAD4 and Ski, followed by the corresponding secondary antibodies labeled with fluorescein isothiocyanate (green) for Ski and Cy3 (red) for SMAD4. Both Ski and SMAD4 localized in the nuclei of HSCs; yellow fluorescence indicates colocalization. After administration of acetaldehyde, both proteins are exported to the cytosol (arrow). However, a fair amount of SMAD4 is present in the nucleus (red). B: Immunoprecipitation (IP) of SMAD4, followed by Western analysis for the detection of Ski in HHSCs cultured in the absence or presence of 200 μmol/L acetaldehyde (B). Results are representative of three independent experiments. Original magnification, ×40.