Figure 8.

NF-κB inhibition reduces UVB-induced cutaneous inflammation and tumorigenesis in Ptch^+/− hairless mice. A: Effects of chronic treatment of mice with 300 ppm of SSZ in drinking water on UVB-induced carcinogenesis. ^∗P < 0.05. B: Semiquantitative RT-PCR analysis of Shh signaling genes in UVB-induced BCCs and tumor-adjacent perilesional skin. C: Western blot analysis showing expression of NF-κB–related signaling proteins in skin/tumors of SSZ-treated and UVB-alone–treated mice. D: Effects of SSZ on the expression of cell cycle regulatory proteins, proliferation marker, PCNA, and apoptosis-related protein, BclII. E: Effects of SSZ on the expression of EMT regulatory proteins. F: PCR array analysis of inflammatory cytokines in the skin of SSZ-treated and UVB-alone–irradiated animals. FLIP, FLICE-inhibitory protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IKK, IκB kinase.