TABLE 2.
RsmA- and RsmZ-dependent production of lipase, pyocyanin, and HCN and swarming ability in P. aeruginosa strains
| Strain (genotype) | Swarminga | Lipaseb (ng/109 cells) | Pyocyaninc (μg/109 cells) | HCNd (μM) |
|---|---|---|---|---|
| PAO1 (wild type) | + | 1.48 ± 0.52 | 1.24 ± 0.21 | 6.25 ± 0.42 |
| PAZH13 (rsmA) | − | 0.77 ± 0.19 | 4.89 ± 0.13 | 32.50 ± 1.88 |
| PAO6354 (rsmZ) | + | 1.69 ± 0.39 | 0.92 ± 0.26 | 5.57 ± 0.92 |
| PAO1/pME6000 (control for pME3337.1) | + | 1.18 ± 0.39 | 0.76 ± 0.08 | 5.28 ± 1.32 |
| PAO1/pME3337.1 (rsmZ++) | − | 0.21 ± 0.02 | 5.92 ± 0.98 | 16.53 ± 0.98 |
a
Swarming ability was tested on semisolid medium incubated at 37°C, as described in Materials and Methods. +, ability to swarm and completely invade the plate within 24 h; −, no swarming.
b
Concentrations of lipase were measured for strains grown in YEA medium to about 4 × 109 cells ml−1 (see Materials and Methods).
c
Pyocyanin was extracted and quantitated for P. aeruginosa strains grown in glycerol-alanine medium (see Materials and Methods).
d
Concentrations of HCN were determined for strains grown under semianaerobic conditions in MMC medium to about 1.1 × 109 cells ml−1, as described in Materials and Methods.