FIGURE 2.

Perfluorocarbon mounting media used with three modes of microscopy. (A–C) LSCM images captured from 1 μm resolution Z-stacks taken of samples mounted in H₂O, PFD and PP11 respectively. Images were generated by using the “cut” function in Zeiss LSM Image Browser software, where the plane presented represents a cut through the entire z-stack from top to bottom taken at an angle of -24°. The images therefore show a progression through the stack from top to bottom. Signal was collected from 518 to 604 nm for Venus, and is displayed in green and at 647–690 nm for chlorophyll, shown in red. (D–F) 2-photon micrographs taken of samples mounted in H₂O, PFD, and PP11 respectively. (G–I) SHG micrographs taken of samples mounted in H₂O, PFD, and PP11 respectively. (D–I) are presented as projections of z-stacks. Scale bars are 50 μm.