Table 2.
qRT-PCR primer sequences.
| Gene | Assay name (Qiagen) | Primer assay for mature miRNA sequence (5′–3′) |
|---|---|---|
| miR-25 | Bt_miR-25_1 | CAUUGCACUUGUCUCGGUCUGA |
| miR-302c | Hs_miR-302c_1 | UAAGUGCUUCCAUGUUUCAGUGG |
| miR-196A1 | Hs_miR-196a_2 | UAGGUAGUUUCAUGUUGUUGGG |
| miR-181A1 | Hs_miR181a_2 | AACAUUCAACGCUGUCGGUGAGU |
| miR-370 | Hs_miR-370_1 | GCCUGCUGGGGUGGAACCUGGU |
| miR-39* | Ce_miR-39_1 | UCACCGGGUGUAAAUCAGCUUG |
| NOBOX (Tripurani et al., 2011) | Forward: | AGACCCAGTTCTTCCAACACC |
| Reverse: | CACAGGACAAGGCAAAGAGAG | |
| GAPDH** | Forward: | TGCCCAGAATATCATCCC |
| Reverse: | AGGTCAGATCCACAACAG |
*
Housekeeping gene/internal control for culture media.
**
Housekeeping gene for embryos.