Table 2.

CTG size information obtained from Triplet-repeat Primed PCR, conventional PCR and Southern Blot.

	Results from Triplet Primed PCR			Results from conventional PCR and Southern Blot
Sample ID	CTG number, FAM labeled-forward primer	CTG number, HEX labeled-reverse primer	Detection of expanded allele	CTG number, FAM labeled-forward primer	CTG number, convention PCR with reflex Southern Blot
DM-1	5	4	No	5	5
DM-2	5/85*	4/84*	Yes	5/85*	5/85*
DM-3	5	4	Yes	5	5/700*
DM-4	11/12	10/11	No	11/12	11/12
DM-5	13/14	12/13	No	13/14	13/14
DM-6	12	11	No	12	12
DM-7	13	12	No	13	13
DM-8	13	12	No	13	13
DM-9	21	20	No	20	20
DM-10	12/13	11/12	No	12/13	12/13
DM-11	5	4	Yes	5	5/1300–1700*
DM-12	12/13	11/12	No	11/12	11/12
DM-13	12	11	Yes	11	11/700–1700*
DM-14	14	13	Yes	13	13/1800*
DM-15	5	4	Yes	4	4/150–450*
DM-16	11/12	10/11	No	10/11	10/11
DM-17	11/13	10/12	No	11/12	11/12
DM-18	12/13	11/12	No	10/11	10/11
DM-19	5	4	No	4	4
DM-20	10/16	9/15	No	10/16	10/16
DM-21	12	11	Yes	12	12/350*
DM-22	5/12	4/11	No	5/12	5/12
DM-23	12	11	Yes	12	12/550*
DM-24	12/13	11/12	No	12/13	12/13
DM-25	10/16	9/15	No	10/16	10/16
DM-26	21	20	Yes	21	21
DM-27	5/13	4/12	No	5/13	5/13
DM-28	12/13	11/12	No	12/13	12/13
DM-29	12	11	Yes	11	11/380*
DM-30	5/11	4/10	No	5/10	5/10
DM-31	5/12	4/11	No	4/11	4/11
DM-32	5/13	4/12	No	4/12	4/12
DM-33	12	11	Yes	11	11/600*
DM-34	5/13	4/12	No	4/12	4/12
DM-35	10/16	9/15	No	9/15	9/15
DM-36	12/13	11/12	No	11/12	11/12
DM-37	12	11	Yes	11	11/350*
DM-38	10/16	9/15	No	9/15	9/15
DM-39	13/20	12/19	No	12/19	12/19
DM-40	12/14	11/13	No	11/13	11/13
DM-41	12	11	Yes	11	11/409*
DM-42	12	11	Yes	11	11/350*
DM-43	5/11	4/10	No	4/10	4/10
DM-44	5	4	No	4	4
DM-45	12	11	Yes	11	11/634*

^*indicates an expanded allele.