TABLE 1.
Reduction of Fe(III)NTA, AQDS, and fumarate by cell suspensions
| Suspensiona and electron donor | Mean productionc (μmol/mg/min) ± SD
|
||
|---|---|---|---|
| Fe(II)-NTA | AHQDSb | Succinate | |
| Wild type | |||
| Acetate | 0.84 ± 0.17 | 0.64 ± 0.03 | 0.182 ± 0.002 |
| Hydrogen | 1.48 ± 0.22 | 1.29 ± 0.19 | 0.091 ± 0.010 |
| Hya deficient | |||
| Acetate | 1.24 ± 0.05 | 0.81 ± 0.01 | 0.189 ± 0.001 |
| Hydrogen | 1.83 ± 0.10 | 1.46 ± 0.05 | 0.072 ± 0.013 |
| Hyb deficient | |||
| Acetate | 0.95 ± 0.04 | 0.62 ± 0.05 | 0.187 ± 0.011 |
| Hydrogen | 0.00 ± 0.03 | 0.02 ± 0.01 | 0.004 ± 0.004 |
a
Resting cell suspensions were prepared from late-log-phase acetate-fumarate cultures as described in Materials and Methods.
b
AHQDS is the reduced form of AQDS.
c
Data are means of triplicate incubations, except for measurements of wild-type reduction of AQDS and Fe(III)-NTA, which are means of six incubations.