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. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Nature. 2014 Apr 9;508(7497):526–530. doi: 10.1038/nature13242

Figure 3. Amoebic trogocytosis contributes to human cell killing.

Figure 3

a – d, Imaging flow cytometry analysis with amoebae expressing a tetracycline (tet)-inducible copy of EhC2PK K179A. a, Measurement of fragmentation. b, Detection of dead cells. c – d, Means and standard deviations for biological duplicates at each time point (10,000 events/each). e, Western blot demonstrating EhC2PK over-expression in tet-induced cells; Lgl, loading control. Blots are representative of three independent experiments. f – g, Imaging flow cytometry analysis with vector control amoebae; shown are means and standard deviations for biological duplicates (10,000 events/each). P-values from t-tests: * < .05, ** < .01, *** < .001.