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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Nature. 2013 Jul 17;500(7463):468–471. doi: 10.1038/nature12313

Figure 3. Rapamycin-induced hMOF downregulation promotes deacetylation of H4K16.

Figure 3

Rapamycin treatment (48 h) promoted the downregulation of the H4K16 histone acetyltransferase hMOF expression level in MEF (a) and transfected HeLa cells (b). VPA (1mM) treatment counteracted rapamycin-induced H4K16ac downregulation (c) and decreased the LC3 ratio (d). (e) Cotreatment with chloroquine (CQ, 10 μM) showed that the decrease in LC3 ratio was a result of an increase in autophagic flux. (f) Inhibition of autophagy by CQ after hMOF overexpression shows that hMOF does not inhibit autophagic flux. (g) The yeast homolog of hMOF, Sas2, tagged with 3xHA showed a complete disappearance of HA signal upon autophagy induction after 3 h. (h) Overexpression of Sas2 repressed the downregulation of H4K16ac upon rapamycin treatment.