Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Proteins. 2013 Nov 22;82(5):867–878. doi: 10.1002/prot.24464

Figure 7.

Figure 7

Size exclusion chromatograms for native disulfide-intact A1 (closed symbols) and RCAM A1 (open symbols) in buffer (circles) and 0.25M GdnHCl (squares). The column was equilibrated with PGA buffer and calibrated with several proteins as indicated by their peak maxima (a - Ovoalbumin from chicken (44.0kDa), b - bovine Carboanhydrase (29kDa), c - Myoglobin from horse (17.0kDa), d cytochrome c from horse (12.4kDa). Void volume = 6.85mL with blue dextran (2000kDa). Included volume 17.54mL with vitamin B12 (1.35kDa).