Alignment of amino acids among Mrp family proteins. The numbers indicate the positions of amino acids starting from the N-terminal end of each protein. The amino acids marked by black and gray boxes indicate identical and similar amino acids, respectively. Accession numbers in PIR; B. subtilis, A69743; Staphylococcus aureus, A90012; Caulobacter crescentus, F87508; Halobacterium, H84268; Arabidopsis thaliana, T06147; Homo sapiens, AK022722. Multiple alignment was carried out by CLUSTAL W (47), and the figure was made by BOXSHADE. The brackets marked by W and M under the alignment indicate the conserved Walker-motif and the MRP signature, respectively (51; BSORF Web site [http://bacillus.genome.ad.jp]).