Inhibition of aprE-lacZ expression by salA disruption, and recovery by intact or mutant salA on plasmid pDG148. Cells were grown in Schaeffer's sporulation medium, and β-galactosidase activities were determined as described in Materials and Methods. Data are from one of two experiments. Numbers on the x axis represent the growth time in hours relative to the end of the vegetative growth (T0). ○, OAM145 (WT) carrying pDG148; •, OAM151 (salA) carrying pDG148; □, OAM145 (WT) carrying pDG-salA; ▪, OAM151 (salA) carrying pDG-salA; ▴, OAM151 (salA) carrying pDG-salA-m.