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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Cell Signal. 2014 Mar 6;26(6):1303–1309. doi: 10.1016/j.cellsig.2014.02.018

Fig. 1.

Fig. 1

Effects of rottlerin on Wnt3A and LRP6-induced Wnt/β signaling in HEK293 cells. (A, B) HEK293 cells in 24-well plates were transiently transfected with LRP6 plasmid or the corresponding control vector, along with Super8XTOPFlash construct and β-galactosidase-expressing vector in each well. After being incubated for 24 h, cells were treated with rottlerin (A) or rottlerin plus Wnt3A CM (B) at indicated concentrations for 24 h. The luciferase activity was then measured 24 h later with normalization to the activity of the β-galactosidase. Values are averages of three determinations with the standard deviations indicated by error bars. **P < 0.01 compared to the control cells without niclosamide treatment. (C) HEK293 cells in 6-well plates were treated with rottlerin (4 μM) for 24 h. The levels of cytosolic free human β-catenin (92 kDa), total cellular human β-catenin (92 kDa), LRP6 (210 kDa for mature form) and phospho-LRP6 (p-LRP6, 210 kDa)) were examined by Western blotting. All the samples were also probed with anti-human actin antibody to verify equal loading.