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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Cell Signal. 2014 Mar 6;26(6):1303–1309. doi: 10.1016/j.cellsig.2014.02.018

Fig. 2.

Fig. 2

Effects of rottlerin on Wnt/β-catenin signaling in cancer cells. Prostate cancer PC-3 and DU145 cells and breast cancer MDA-MB-231 and T-47D cells in 6-well plates were treated with rottlerin at the indicated concentrations for 24 h. The levels of cytosolic free human β-catenin (92 kDa), total cellular human β-catenin (92 kDa), human LRP6 (210 kDa for mature form), phospho-LRP6 (p-LRP6, 210 kDa)) and axin2 (95 and 98 kDa) were examined were examined by Western blotting. All the samples were also probed with anti-human actin antibody to verify equal loading.