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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Cell Signal. 2014 Mar 6;26(6):1303–1309. doi: 10.1016/j.cellsig.2014.02.018

Fig. 5.

Fig. 5

Rottlerin suppresses LRP6 expression at the transcription level. (A) Prostate cancer PC-3 cells and breast cancer T-47D cells were treated with rottlerin at the indicated concentrations for 24 h. Total RNA was extracted from the indicated cell lines, and LRP6 mRNA levels were determined by real-time RT-PCR and normalized to the message levels of GAPDH mRNA. All the values are the average of triple determinations with the s.d. indicated by error bars. (B) Cancer cells were transiently transfected with the LRP6 promoter plasmid, or the actin promoter reporter plasmid. After being incubated for 24 h, cells were treated with rottlerin at the indicated concentrations. The luciferase activities were then measured 24 h later, and the LRP6 promoter luciferase activity was normalized to the actin promoter luciferase activity. All the values are the average of triplicate determinations.