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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Cell Signal. 2014 Mar 6;26(6):1303–1309. doi: 10.1016/j.cellsig.2014.02.018

Fig. 7.

Fig. 7

Effects of rottlerin on mTORC1 signaling in cancer cells. Prostate cancer PC-3 and DU145 cells and breast cancer MDA-MB-231 and T-47D cells in 6-well plates were treated with rottlerin at the indicated concentrations for 24 h. The levels of human P70-S6K (70 kDa), phospho-P70-S6K (70 kDa), S6 (32 kDa) and phospho-S6 (32 kDa) were examined by Western blotting. All the samples were also probed with anti-actin antibody to verify equal loading.