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TtgV repression of the transcription of PttgG is alleviated by 1-hexanol. Single-round in vitro transcription assays were performed as described in the legend to Fig. 1A in the absence and presence of 0.5 μM TtgV added before the addition of RNA polymerase. Increasing concentrations of 1-hexanol were added as indicated. Other conditions as are described in Materials and Methods.
