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In vitro methylation protection assay of the ttgGHI promoter region. PCR fragments comprising the ttgG promoter region were labeled at one 5′ end and incubated without (−) and with 1 or 3 μM purified TtgV before being subjected to treatment with DMS or DNase I digestion. The positions of the G's that are protected from methylation by the repressor are indicated; the asterisk indicates a hyperreactive position.
