TABLE 1.

Oligonucleotides used for gene knockouts

Gene and orientation	Sequence (5′ → 3′)
arsA reverse	CGGTCACCTCAGTGATCGTC
arsA inverse	CTGCGAATCCCCTGTCGAGACGA
arsC inverse	GCGCGTTGTAGACCTGTTCG
arsC reverse	AACAGACACCACCGGAACGC
2599 reverse	CTACGCGAAGAACTGTGCCA
arsB 5′ inverse	CGCTGACATGGTGCCACGGT
arsB 3′ inverse	TGGGGCGTCTAAGGCGGATA
2603 forward	ATGGAACAAGTGTGGGCAGA
5175 reverse	CTATTTGAAATCCTGAAAGA
arsM 3′ inverse	GACTGACCGGCCGAATCG
arsM 5′ inverse	CATGGTTGCCTCGCAGGT
5176 forward	ATGGTTCAGGACGCAACCGC
ura3 forward	ATGAGCTTCGTCGAGGAACT
ura3 reverse	CTACCGGTGGCGGTTCAGGC
bla forward	ATGAGTATTCAACATTTCCG
bla reverse	TTACCAATGCTTAATCAGTG
tbpC forward	ATGACGGTCGAGATTGCGAA
tbpC reverse	TTAAACTAATTCTTGGACTT