Figure 3.

Cell density and proliferation. (a) The area fraction occupied by cell nuclei after 1 h in culture, as a measure of initial cell density, is plotted against the island width. None of the differences between conditions is statistically significant. Data represent mean ± s.d., n = 3. (b) The percentage of proliferating cells at 48 h of culture on adhesive islands, as identified by incorporation of EdU during 4 h of culture, is plotted against the island width. None of the differences between conditions is statistically significant. Data represent mean ± s.d., n = 4. (c) Fluorescent micrographs of cells cultured on adhesive islands for 48 h, stained for incorporation of EdU (red) and cell nuclei (blue) after 4 h of incubation with EdU. (Online version in colour.)