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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Lab Invest. 2014 Mar 10;94(5):503–516. doi: 10.1038/labinvest.2014.42

Figure 2. Leptin dose-dependently and differentially regulated gene expression of RAGE and AGE-R1 in activated HSC in vitro.

Figure 2

Serum-starved HSC were treated with leptin (100 ng/ml) as indicated. Total RNA or whole cell extracts were prepared. A. Real-time PCR analyses of RAGE and AGE-R1 mRNA. Values were expressed as mRNA fold changes (means ± SD) (n= 3). *P<0.05 vs. the untreated control (the corresponding 1st column). B. Western blotting analyses of RAGE and AGE-R1. β-actin was used as an invariant control for equal loading. Representatives were presented from three independent experiments.