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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Lab Invest. 2014 Mar 10;94(5):503–516. doi: 10.1038/labinvest.2014.42

Figure 8. Forced expression of Nrf2, or dn-Nrf2, cDNA showed opposite impacts on the regulation of the promoter activities of RAGE and AGE-R1 genes in HSC.

Figure 8

Passaged cells were co-transfected with the luciferase report plasmid pRAGE-luc (A), or pAGE-R1-luc (B), and the cDNA expression plasmid pNrf2 (A), or pdn-Nrf2 cDNA (B), at various doses plus the empty vector pcDNA. The latter was used to ensure equal amount of total DNA in the co-transfection. After recovery, cells were serum-starved for 4 h and treated with or without AGEs (100 μg/ml) (A), or curcumin (Cur) (20 μM) (B), in serum-depleted DMEM for 24 h. Luciferase activities were analyzed and expressed as relative units after normalization with β-galactosidase activities (means ± SD, n=6). *P<0.05 vs. the untreated control cells (the corresponding 1st column). ‡P<0.05 vs. the cells treated with AGEs (A), or curcumin only, (the corresponding 2nd column) (B). The insets denoted the co-transfection of HSC with a luciferase reporter plasmid plus a cDNA expression plasmid in the system.