FIG. 1.
Northern blot analysis of cid operon transcription in S. aureus RN6390 and UAMS-1. Ten-microgram samples of total RNA were collected at 2, 6, and 12 h, separated in a 1.0% formaldehyde-agarose gel, and hybridized to cidB- and cidC-specific DIG-labeled probes. The sizes of the 2.7- and 2.2-kb transcripts were determined by comparison to an RNA ladder (Invitrogen) run on the same gel. The corresponding ethidium bromide-stained gels are shown underneath each blot.
