TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| Serovar Typhi strains | ||
| IMSS-1 | Mexican clinical isolate; 9,12, d:-, Vi | 42 |
| 81 | Serovar Typhi ΔompB::km isogenic IMSS-1 derivative | 31 |
| IMSS-40 | Serovar Typhi ΔompR::km isogenic IMSS-1 derivative | This study |
| IMSSTN103 | Serovar Typhi isogenic IMSS-1 derivative containing a Tn10::Cm insertion 160 bp upstream of the first ATG and 60 bp downstream of the promoter for the leuO gene | This study |
| Plasmids | ||
| pFM413S2 | pBR322-derived plasmid, containing the whole structural ompS2 gene plus its 5′ regulatory region, up to 413 bp upstream of the transcriptional start point | This study |
| pFM97S2 | pBR322-derivative, containing the ompS2 gene plus the regulatory region up to position −97 | This study |
| pFM413 to 34 series | pMC1871-derived plasmids containing translational fusions of the ompS2 5′ regulatory region, up to 413 to 34 bp upstream of the transcriptional start point, to the lacZ reporter gene | This study |
| pMPM-A3 | p15A-derived vector (Apr), with a multiple cloning site | 32 |
| pFM800TL | pMPMA-3-derived plasmid containing the serovar Typhi leuO gene together with Tn10 inserted in the upstream region | This study |
| pFMTrcleuO | pFMTrc12 (p15A1 lacI trcp Apr)-derived plasmid containing serovar Typhi leuO | This study |
| pKD46 | Red recombinase expression plasmid (Apr) | 10 |
| pKD4 | Template plasmid for gene inactivation with Kmr | 10 |