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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Lab Invest. 2014 Mar 10;94(5):545–556. doi: 10.1038/labinvest.2014.43

Figure 5. Smad3 deficiency inhibits collagen I and fibronectin expression in the kidney.

Figure 5

A. The mRNA levels of collagen I in the kidney of WT and Smad3-KO mice as determined by real-time RT-PCR. * P < 0.05 vs WT controls, + P < 0.05 vs KO UUO, and # P < 0.05 vs WT UUO. n=4 per group. B. The mRNA levels of fibronectin in the kidney of WT and Smad3-KO mice as determined by real-time RT-PCR. * P < 0.05 vs WT controls, + P < 0.05 vs KO UUO, and # P < 0.05 vs WT UUO. n=4 per group. C. Representative photomicrographs of collagen I immunofluorescence staining in the kidney of WT and Smad3-KO mice at day 14 after UUO (original magnification X400). D. Representative photomicrographs of fibronectin immunofluorescence staining in the kidney of WT and Smad3-KO mice at day 14 after UUO (original magnification X400). E. Representative Western blots show the protein levels of collagen I in the kidney of WT and Smad3-KO mice. F. Quantitative analysis of collagen I protein expression in the kidney of WT and Smad3-KO mice. * P < 0.05 vs WT controls, + P < 0.05 vs KO UUO, and # P < 0.05 vs WT UUO. n=4 per group. G. Representative Western blots show the protein levels of fibronectin in the kidney of WT and Smad3-KO mice. H. Quantitative analysis of fibronectin protein expression in the kidney of WT and Smad3-KO mice. * P < 0.05 vs WT controls, + P < 0.05 vs KO UUO, and # P < 0.05 vs WT UUO. n=4 per group.